Nitrifying bacteria immobilized on Fe2O3-CaO-SiO2 porous glass-ceramics have extensive application prospects for wastewater treatment. to study the effects of the pore structure on the immobilization, Fe2O3-CaO-SiO2 porous glass-ceramics with different porosities and pore sizes were prepared by sintering and the addition of pore-forming agent. The pore size and apparent porosity can be controlled by the pore-forming agent content and their sizes. Appropriate amount of the pore-forming agent can promote the apparent porosity and increase the amount of immobilized nitrifying bacteria. The excess of the pore-forming agent can cause the formation of the new crystalline phases and reduce the amount of immobilized nitrifying bacteria.