Quantitative Detection Of Eight Pthalate Metabolites In Human Urine Using Hplc-Apci-Ms/Ms

Because of the ubiquity of phthalates and their potential role in increasing risk for cancer and reproductive dysfunction, the need for human exposure assessment studies is urgent. In response to this need, a high-throughput, robust, sensitive, accurate and precise assay for simultaneous measurement of trace levels of eight phthalate metabolites in human urine by HPLC-MS/MS is developed. Human urine samples are processed using enzymatic deconjugation of the glucuronides followed by solid-phase extraction. The evaluate is concentrated, and the phthalate metabolites are chromatographically resolved by reversed-phase HPLC, detected by APCI-tandem mass spectrometry, and quantified by isotope dilution.