Due to its excellent bioactivity, bioactive glass (BG) is suitable for use as bone graft substitutes in biomedical applications. In this study, carbon nanotubes (CNT-COOH) served as templates for depositing bioactive glass based on 60SiO2-36CaO-4P2O5 wt% were synthesized via the solgen process. The BG and BG/CNT-COOH composites were treated at 300, 500, 700 and 900 deg C; their properties were also examined by XRD, Fourier transform infrared spectroscopy, and SEM. Experimental results showed that BG/CNT-COOH composites treated at 500 and 700 deg C were amorphous and contained silicate nanocrystals. By altering precursor concentration, bioactive glass of various thicknesses was successfully sol-gel coated on CNT-COOH. Immersion of the BG/CNT-COOH composites in simulated body fluid solution and MG-63 cell culture assessment showed the 500 deg C treated BG/CNT-COOH exhibits excellent bioactivity.